Abstract

Background: One of the most significant diseases for swine, including domestic pigs and wild boars, in terms of both public health and economics is brucellosis. This disease, which is brought on by the bacterium Brucella suis (B. suis), is regarded as a zoonotic disease that can spread from animals to humans and is a serious public health risk. The most recent report on the prevalence of swine brucellosis in the Philippines was published in the 1990s, thus there is neither updated nor recent data available to the public. Thus, the purpose of this investigation was to detect B. suis in pigs in selected provinces in Luzon, Philippines using qPCR and ELISA. Methods: To detect B. suis antigen and antibody, 115 blood samples of pigs from 15 slaughterhouses spread across 8 provinces were gathered and subjected to qPCR and ELISA. Results: B. suis DNA and anti- Brucella LPS antibodies were not detected in any of the 115 blood samples as demonstrated by qPCR and ELISA tests, respectively. Conclusions: In general, this study’s findings offered updates on the B. suis infection status, indicating that pigs from the 8 provinces of Luzon, Philippines, did not have the B. suis chromosome 2 biovar 1 gene in their blood.

Keywords

ELISA, qPCR, swine brucellosis, B. suis

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